In addition to the standard baking pad length of 10 mm, we also offer pad lengths ranging from 1 mm to 100 mm. Light paths smaller than 1 mm are also available, but are for more specialized cells, such as detachable buckets or HPLC flow buckets. Mix the sample well before use, especially when nucleic acids or proteins are stopped again. Avoid using glass pipettes to fill the bucket, as these can cause scratches on the optical surface.
It is not recommended to use plastic buckets on UV fish spectrometers. Technically, any standard 10×10mm bucket can be used, but you need to know the material and transmission profile before doing so. Around 300 nm, even UV plastic buckets begin to absorb light and affect the results.
Excellence buckets provide exceptional window parallelism and offer small optical path length tolerances of just ± 0.01 millimeters. This enables them to deliver the precision needed for pharmaceutical and research applications. Made of JGS1 UV grade molten silica, the onset of absorption occurs below 170 nm and there are no absorption bands between 170 nm and 250 nm. Exceptionally high transmission over UVB, UVA, visible spectrum and near IR. The transmission spectra of our UV quartz cells can be seen below.
You get the affordable full range of UV quartz material, the $12.3 price tag for QS2002 makes it a great buy for labs on a budget. For UV-VIS absorption studies you absolutely need a UV quartz tammmer, if you cut corners here when getting a cheap glass or plastic cell, your data will be inaccurate, it is not advisable to do this. This baking material is suitable for the visible spectrum and has a decent transmission range of 340-2,500 nm. In this article, we will introduce the basic material requirements, and then we will draw a contrast between the different types of buckets to help you select the right bucket.
Run the solution on the glass wall towards the bucket to prevent bubble formation. In a standard spectrometer box, the path of the light or the length of the path is the internal distance from the front window to the rear window. The length of the optical path of the standard bucket is 10 mm. Both standard and excellence buckets are designed for long-term use.
However, there is a chance that the samples are too diluted and it is not possible to obtain sufficient absorption with standard-sized buckets. Of course, the solution can concentrate the samples, but it is usually difficult in situations where the sample evaporates or undergoes a chemical change during the concentration process. The light path or path length of a typical spectrophotometer box is the internal distance from the front window to the rear window of the transparent walls of the bucket. With a wall thickness of 1.25 mm, the standard outer dimensions of a bucket are 12.5 x 12.5 m, height 45 mm.
For example, if a photometer with a linear measuring range up to 2 A with a path length of 10 mm is used, the double-stranded DNA can be reliably quantified to a maximum concentration of 100 μg/ml. Solutions of higher concentrations must be diluted or dilution can be simulated Cuvettes using a cuvette with a shorter path length. According to Lambert-Beer’s law, a path length of 1 mm makes it possible to measure dsDNA concentrations up to 1,000 μg/ml. If only a small amount of sample is available, reuse of the sample for later measurements may be considered.
Plastic buckets, if packaged separately and of an appropriate degree of purity, will minimize the risk of contamination. Alternatively, buckets designed to handle extremely small volumes can be selected. If the sample is based on an aqueous solution, then the material from which the bucket is made is relatively insignificant. If, on the other hand, they are organic solvents, glass buckets have the preferred option, as they show greater strength compared to variants of plastic. For these reasons, certain buckets, such as macro buckets, offer an advantage in temperature-controlled applications.
All these materials seem absolutely clear and suitable for all kinds of absorption studies at first glance. However, each material has different light-absorbing properties and it is vital to take these optical characteristics into account when choosing a bucket material. The most popular UV fish buckets refer to buckets with rectangular test tubes, made of quartz material, for use in UV visspectroscopic measuring systems. They are used, like standard test tubes, to store aqueous solutions.
Since plastic buckets are used only once and do not need to be cleaned, there is no need to take into account possible damage and loss. They are an excellent choice for fake hellometry and spectrophotometry. There is a negligible variation in absorption between the four optically bright sides, so that the measurement can be taken from both sides.